Genetic transformation methods for transient protein expression

10 Novembro 2021, 08:00 • Susana Maria Traquete Serrazina

Concept of transient expression. Preparation of genetic transformation vectors with the gene of interest associated to the reporter gene, focusing the expression cassette. Characteristics of the reporter proteins GUS and GFP. Agrobacterium as a genetic transformation agent. Agroinfiltration as a eukaryotic genetic transformation system: in vivo preparation of the infiltration solution with the transformation agent and infiltration of Nicothiana benthamiana leaves. Biolistics as a eukaryotic and prokaryotic transformation system: demonstration of microparticle coating, bombardment of N. benthamiana leaves, and visualization of GUS reporter protein expression.