Sumários
Análise dos clones transformantes
13 Abril 2018, 14:00 • Rita Zilhão
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Análise dos clones transformantes
Análise e interpretação dos resultados obtidos na transformação de E. coli.
Repicagem dos transformantes para caixas de meio seletivo Amp/X-gal.
Extração de DNA plasmídico dos transformantes, a partir de culturas de 18h de incubação.
Análise dos clones transformantes
13 Abril 2018, 10:30 • Rita Zilhão
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Análise dos clones transformantes
Análise e interpretação dos resultados obtidos na transformação de E. coli.
Repicagem dos transformantes para caixas de meio seletivo Amp/X-gal.
Extração de DNA plasmídico dos transformantes, a partir de culturas de 18h de incubação.
Continuação do tópico 4, e 5. Técnica de PCR e variantes
13 Abril 2018, 08:00 • Rita Zilhão
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Continuação sw sistemas de expressão génica induzível e condicional. Sistema tet-on/tet-off e sistema cre-lox.
5. Técnica de PCR e variantes
História do PCR. Princípios da técnica de PCR convencional e análise dos seus produtos. Características das polimerases de DNA utilizadas em PCR. A escolha dos primers. Estratégias de clonagem de produtos de PCR. Variantes da técnica de PCR e suas aplicações: a síntese de cDNA e a RT-PCR, Nested-PCR, sistema ARMS, DOP-PCR e PCR multiplex. Técnicas de mutagénese dirigida baseada em PCR (PCR invertido e SOE-PCR (Splicing by overlapping extension). A utilidade do PCR em protocolos de screening.
Análise dos clones transformantes
10 Abril 2018, 17:00 • Rita Zilhão
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Análise dos clones transformantes
Análise e interpretação dos resultados obtidos na transformação de E. coli.
Repicagem dos transformantes para caixas de meio seletivo Amp/X-gal.
Extração de DNA plasmídico dos transformantes, a partir de culturas de 18h de incubação.
Análise dos clones transformantes
10 Abril 2018, 09:00 • Rita Zilhão
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Análise dos clones transformantes
Análise e interpretação dos resultados obtidos na transformação de E. coli.
Repicagem dos transformantes para caixas de meio seletivo Amp/X-gal.
Extração de DNA plasmídico dos transformantes, a partir de culturas de 18h de incubação.