TRATAMENTO DE DADOS OBTIDOS COM A CÂMARA DE USSING

25 Outubro 2022, 17:00 • Ines Pankonien

·      Quantify the electric current generated by the ion transport that was measured in the Ussing chamberclass PL4

·      Through the quantifications the effect of the drugs used for the rescue of CFTR mutation F508del in the previous class was compared 

·      How the Ussing chamber measurements can predict the best treatment for a person with Cystic Fibrosis

·      The Transepithelial voltage measured in the Ussing chamber and the small current pulses (5μA/s) injected were used to calculate the Transepithelial Resistance using Ohm‘s law: R=V/I

·      To calculate the electric current generated by the ion transport, we apply Ohm’s law: Isc = Vte / Rte 

·      The current (Isc) that passes between two sides is directly proportional to the voltage (Vte) and inverse to the resistance (Rte) between these sides

Mini Quiz 2 

·      Test the knowledge and understanding related to the epithelial ion channel classes (PL3, PL4, PL5)