Módulo M&S2 (Elsa Anes): Legionella pneumophila outer membrane vesicles isolation and analysis of their pro-inflammatory potencial on macrophages

30 Abril 2020, 18:00 • Elsa Anes

 Legionella pneumophila outer membrane vesicles isolation and analysis of their pro-inflammatory potencial on macrophages

1. OMVs concept, applications
   
2. Cultivate L.p and prepare and quantify L.p outer membranes vesicles (OMVs)
   
3. Pre-treatment of macrophages, infection and assessment of bacterial intracellular survival with a colony-forming Unit (CFU) assay
   
4. Results: pro-inflammatory response of macrophages from Lp infection
   
5. Conclusions