Protein Expression, Cell lysis, Fraction Preparation and Protein Purification Miniscreens

15 Novembro 2019, 14:00 • Manuela Pereira

Protein Expression, Cell lysis, Fraction Preparation and Protein Purification Miniscreens

1.       Protein expression

2.       Protein expression in E. coli

3.       Cell lysis

4.       Fraction preparation

5.       Protein purification

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Module 4: Protein Biophysics and Structural Biochemistry (Cont.)

15 Novembro 2019, 14:00 • Cláudio Emanuel Moreira Gomes

Module 4: Protein Biophysics and Structural Biochemistry (Cont.) Preparation of protein samples. CD analysis of proteins. Preparation of protein samples and experiments designed by the students

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Gene Cloning

8 Novembro 2019, 14:00 • Manuela Pereira

Gene Cloning

1.Basic molecular cloning of DNA and protein expression

2. Get your gene of interest cloned

3. Introducing the plasmid in a host

4. Select transformed strain and confirm your clone

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Module 4: Protein Biophysics and Structural Biochemistry.

8 Novembro 2019, 14:00 • Cláudio Emanuel Moreira Gomes

Module 4: Protein Biophysics and Structural Biochemistry. Preparation of protein samples. Circular Dichroism analysis of proteins and thermal stability measurements. Theoretical background and analysis of scientific literature. 

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Tutorial on Molecular Docking using the AutoDock suite

25 Outubro 2019, 14:00 • Miguel Machuqueiro

The process of drug discovery takes years to decades until a new drug is found, while also being very costly. Efforts to cut down the research timeline and costs by reducing lab experiments led to the use of computational modeling and, in particular, molecular docking calculations. In this tutorial, we will use the Autodock software to predict how small molecules, such as substrates or drug candidates, bind to a receptor of known 3D structure. AutoDock has been widely-used and there are many examples of its successful application in the literature, including its application to AChE inhibitors, used here. We will test a strong AChE inhibitor (donepezil) and a milder compound (rosmarinic acid).

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