Project development – Structural Biochemistry

28 Outubro 2022, 09:00 • Cláudio Emanuel Moreira Gomes

Project development – Structural Biochemistry (Protein folding studies, spectroscopic analysis of Protein interactions, Pull-Down assays)

Scale up, Protein Purification and Evaluation

21 Outubro 2022, 14:00 • Manuela Pereira

 Protein purification

Protein chromatography

Chromatography systems

Buffer Design

Advantage of Tags

Protein concentration

Dialysis

Quality control

Scale up, Protein Purification and Evaluation

21 Outubro 2022, 09:00 • Manuela Pereira

Protein purification

Protein chromatography

Chromatography systems

Buffer Design

Advantage of Tags

Protein concentration

Dialysis

Quality control

Protein Expression, Cell lysis, Fraction Preparation and Protein Purification

14 Outubro 2022, 14:00 • Manuela Pereira

1.       Protein expression

2.       Protein expression in E. coli

3.       Cell lysis

4.       Fraction preparation

5.       Protein purification

Protein Expression, Cell lysis, Fraction Preparation and Protein Purification

14 Outubro 2022, 09:00 • Manuela Pereira

1.       Protein expression

2.       Protein expression in E. coli

3.       Cell lysis

4.       Fraction preparation

5.       Protein purification