Sumários
Discussão de artigos
30 Outubro 2023, 09:30 • António Eduardo do Nascimento Ferreira
Discussão de artigos apresentados.
Protein Folding: Fundamental Concepts and Thermodynamics.
23 Outubro 2023, 11:00 • Cláudio Emanuel Moreira Gomes
Protein Folding: Fundamental Concepts and
Thermodynamics. Structural Diversity of Proteins and Introduction to the
Protein Folding Problem. Overview of the Structural Diversity of Proteins.
Protein Folding Problem: Historical Perspective and Current Challenges.
Proteins as Dynamic Entities: Dynamics and Flexibility of Protein Conformation.
Anfinsen's Experiment and the Thermodynamic Hypothesis. Anfinsen's Experiment
and the Thermodynamic Hypothesis of the Protein Folding Process. Definition of
Native and Unfolded States. Kinetics of Protein Folding Reaction: Levinthal's
Paradox and the Concept of Energy Funnel.
Apresentação de artigos
23 Outubro 2023, 09:30 • António Eduardo do Nascimento Ferreira
Apresentação dos artigos 1.3. e 1.4.
Discussão de artigos. Preparação das apresentações.
23 Outubro 2023, 09:30 • Bárbara Joana de Almeida Henriques
Discussão de artigos. Preparação das
apresentações.
Protein structural determination and prediction
16 Outubro 2023, 11:00 • António Eduardo do Nascimento Ferreira
The physical basis of X-ray crystallography. Bragg's law. Applications to biological macro-molecules. Protein crystallization, X-ray sources, diffraction patterns and structure factors. Electron density and structural models. Validation of structural models. The prediction of protein structure (3D) from sequence: a difficult problem in structural biology. The CASP competition. Homology modelling: concept, stages and tools. The quality of the obtained models. Errors and limitations of the results of homology modelling. The portal SwissModel. Template search, modelling and final optimization by empirical force fields. Other methods of structure prediction: the Rosetta fragment approach. The Alphafold method.