Sumários

Aula Prática - Quantification ion transport using the Ussing chamber

24 Outubro 2023, 17:00 Ines Pankonien


·      Analysis of Ussing chamber experiment from previous class

·      Calculate transepithelial resistance (Rte) and short-circuit current (Isc) using Ohm’s Law: V=IR

·      Use LabChart to retrieve all data for calculations in Excel

1.     Measure Vte for all responses to compounds  = transepithelial voltage

2.     Measure ∆Vte for all responses to compounds = ∆ transepithelial voltage

 

·      What is the result? Interpretation of results obtained with the Ussing chamber experiment

·      Mini Quiz 2

Aula Prática - Quantification ion transport using the Ussing chamber

24 Outubro 2023, 16:00 Ines Pankonien



·      Analysis of Ussing chamber experiment from previous class

·      Calculate transepithelial resistance (Rte) and short-circuit current (Isc) using Ohm’s Law: V=IR

·      Use LabChart to retrieve all data for calculations in Excel

1.     Measure Vte for all responses to compounds  = transepithelial voltage

2.     Measure ∆Vte for all responses to compounds = ∆ transepithelial voltage

 

·      What is the result? Interpretation of results obtained with the Ussing chamber experiment

·      Mini Quiz 2

Neurotransmissores e recetores pós-sinápticos

23 Outubro 2023, 14:00 Romana Santos


Tipos de neurotransmissores

Características dos neurotransmissores

Modo de ação dos neurotransmissores

Recetores pós-sinápticos

Canais rápidos sensíveis a ligantes

Canais lentos acoplados à proteína G

Remoção dos neurotransmissores

Como as drogas afetam as sinapses

Plasticidade sináptica

Aula Prática - Measurement of ion transport using the Ussing chamber

18 Outubro 2023, 17:00 Ines Pankonien


·      Ussing chamber technique

·      Analyse ion transport through CFTR, using electrophysiological method such as the Ussing chamber

·      Study how mutations in CFTR ion channel affect channel activity

·      Analyse the effect of pharmacological drugs in the rescue of these mutations

·      Learn how we can use electrophysiology to predict the best drug for a patient with Cystic Fibrosis

Aula Prática - Measurement of ion transport using the Ussing chamber

18 Outubro 2023, 16:00 Ines Pankonien


·      Ussing chamber technique

·      Analyse ion transport through CFTR, using electrophysiological method such as the Ussing chamber

·      Study how mutations in CFTR ion channel affect channel activity

·      Analyse the effect of pharmacological drugs in the rescue of these mutations

·      Learn how we can use electrophysiology to predict the best drug for a patient with Cystic Fibrosis