Sumários
Aula Prática - Quantification ion transport using the Ussing chamber
24 Outubro 2023, 17:00 • Ines Pankonien
· Analysis of Ussing chamber experiment from previous class
· Calculate transepithelial resistance (Rte) and short-circuit current (Isc) using Ohm’s Law: V=IR
· Use LabChart to retrieve all data for calculations in Excel
1. Measure Vte for all responses to compounds = transepithelial voltage
2. Measure ∆Vte for all responses to compounds = ∆ transepithelial voltage
· What is the result? Interpretation of results obtained with the Ussing chamber experiment
· Mini Quiz 2
Aula Prática - Quantification ion transport using the Ussing chamber
24 Outubro 2023, 16:00 • Ines Pankonien
· Analysis of Ussing chamber experiment from previous class
· Calculate transepithelial resistance (Rte) and short-circuit current (Isc) using Ohm’s Law: V=IR
· Use LabChart to retrieve all data for calculations in Excel
1. Measure Vte for all responses to compounds = transepithelial voltage
2. Measure ∆Vte for all responses to compounds = ∆ transepithelial voltage
· What is the result? Interpretation of results obtained with the Ussing chamber experiment
· Mini Quiz 2
Neurotransmissores e recetores pós-sinápticos
23 Outubro 2023, 14:00 • Romana Santos
Aula Prática - Measurement of ion transport using the Ussing chamber
18 Outubro 2023, 17:00 • Ines Pankonien
· Ussing chamber technique
· Analyse ion transport through CFTR, using electrophysiological method such as the Ussing chamber
· Study how mutations in CFTR ion channel affect channel activity
· Analyse the effect of pharmacological drugs in the rescue of these mutations
· Learn how we can use electrophysiology to predict the best drug for a patient with Cystic Fibrosis
Aula Prática - Measurement of ion transport using the Ussing chamber
18 Outubro 2023, 16:00 • Ines Pankonien
· Ussing chamber technique
· Analyse ion transport through CFTR, using electrophysiological method such as the Ussing chamber
· Study how mutations in CFTR ion channel affect channel activity
· Analyse the effect of pharmacological drugs in the rescue of these mutations
· Learn how we can use electrophysiology to predict the best drug for a patient with Cystic Fibrosis