Potencial da membrana em repouso

24 Outubro 2022, 14:00 • Carlos Gravato

Potencial de membrana

Propriedades elétricas passivas das membranas celulares

Resistência e Capacitância

Equação Nernst

Equação de Goldman: potencial de repouso

Mudanças de tensão da membrana: constantes de tempo e comprimento

Membrane potential

Passive electrical properties of cell membranes

  Resistance and Capacitance

Nernst Equation

Goldman Equation: resting potential

Membrane voltage changes: time and length constants

QUANTIFICAÇÃO DO TRANSPORTE IÓNICO UTILIZANDO A CÂMARA DE USSING

19 Outubro 2022, 17:00 • Ines Pankonien

·      Ussing Chamber as a technique to study cellular integrity and the role of ion channels in epithelial transport

·      Measure ion transport through Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) using the electrophysiological method Ussing chamber 

·      Study CFTR function in the cell line CFBE (Cystic Fibrosis Bronchial Epithelial cells) with wt- and F508del-CFTR expression

·      Mutations in the CFTR gene lead to the disease Cystic Fibrosis

·      Study how mutations in CFTR affect this channel activity 

·      Analyze the effect of pharmacological drugs in the rescue of these mutations, and show how the use of electrophysiology/Ussing Chamber can be used to predict the best drug for a patient with Cystic Fibrosis 

·      Pharmacological drugs used are VX-661 and VX-445 (also known as Tezacaftor and Elexacaftor, two CFTR correctors, that help to transport CFTR to the plasma membrane) and VX-770 (also called Ivacaftor, a CFTR potentiator, that maintains the channel open in longer periods of time) 

QUANTIFICAÇÃO DO TRANSPORTE IÓNICO UTILIZANDO A CÂMARA DE USSING

19 Outubro 2022, 16:00 • Ines Pankonien

·      Ussing Chamber as a technique to study cellular integrity and the role of ion channels in epithelial transport

·      Measure ion transport through Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) using the electrophysiological method Ussing chamber 

·      Study CFTR function in the cell line CFBE (Cystic Fibrosis Bronchial Epithelial cells) with wt- and F508del-CFTR expression

·      Mutations in the CFTR gene lead to the disease Cystic Fibrosis

·      Study how mutations in CFTR affect this channel activity 

·      Analyze the effect of pharmacological drugs in the rescue of these mutations, and show how the use of electrophysiology/Ussing Chamber can be used to predict the best drug for a patient with Cystic Fibrosis 

·      Pharmacological drugs used are VX-661 and VX-445 (also known as Tezacaftor and Elexacaftor, two CFTR correctors, that help to transport CFTR to the plasma membrane) and VX-770 (also called Ivacaftor, a CFTR potentiator, that maintains the channel open in longer periods of time) 

Sistema Nervoso | Nervous system

19 Outubro 2022, 15:00 • Carlos Gravato

1- Sistemas de integração: células nervosas e células endócrinas

2- Neurónios

A estrutura celular e as regiões funcionais

Tipos de neurónios

3- Glia

4- Permeabilidade seletiva de membrana e potencial de membrana

1- Integrating Systems: nerve cells and endocrine cells

2- Neurons

  The cellular structure and functional regions

  Types of neurons

3- Glial cells

4- Membrane selective permeability and membrane potential

QUANTIFICAÇÃO DO TRANSPORTE IÓNICO UTILIZANDO A CÂMARA DE USSING

19 Outubro 2022, 12:30 • Ines Pankonien

·      Ussing Chamber as a technique to study cellular integrity and the role of ion channels in epithelial transport

·      Measure ion transport through Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) using the electrophysiological method Ussing chamber 

·      Study CFTR function in the cell line CFBE (Cystic Fibrosis Bronchial Epithelial cells) with wt- and F508del-CFTR expression

·      Mutations in the CFTR gene lead to the disease Cystic Fibrosis

·      Study how mutations in CFTR affect this channel activity 

·      Analyze the effect of pharmacological drugs in the rescue of these mutations, and show how the use of electrophysiology/Ussing Chamber can be used to predict the best drug for a patient with Cystic Fibrosis 

·      Pharmacological drugs used are VX-661 and VX-445 (also known as Tezacaftor and Elexacaftor, two CFTR correctors, that help to transport CFTR to the plasma membrane) and VX-770 (also called Ivacaftor, a CFTR potentiator, that maintains the channel open in longer periods of time)