Sumários
Potencial da membrana em repouso
24 Outubro 2022, 14:00 • Carlos Gravato
Potencial de membrana
Propriedades elétricas passivas das membranas celulares
Resistência e Capacitância
Equação Nernst
Equação de Goldman: potencial de repouso
Mudanças de tensão da membrana: constantes de tempo e comprimento
Membrane potential
Passive electrical properties of cell membranes
Resistance and Capacitance
Nernst Equation
Goldman Equation: resting potential
Membrane voltage changes: time and length constants
QUANTIFICAÇÃO DO TRANSPORTE IÓNICO UTILIZANDO A CÂMARA DE USSING
19 Outubro 2022, 17:00 • Ines Pankonien
· Ussing Chamber as a technique to study cellular integrity and the role of ion channels in epithelial transport
· Measure ion transport through Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) using the electrophysiological method Ussing chamber
· Study CFTR function in the cell line CFBE (Cystic Fibrosis Bronchial Epithelial cells) with wt- and F508del-CFTR expression
· Mutations in the CFTR gene lead to the disease Cystic Fibrosis
· Study how mutations in CFTR affect this channel activity
· Analyze the effect of pharmacological drugs in the rescue of these mutations, and show how the use of electrophysiology/Ussing Chamber can be used to predict the best drug for a patient with Cystic Fibrosis
· Pharmacological drugs used are VX-661 and VX-445 (also known as Tezacaftor and Elexacaftor, two CFTR correctors, that help to transport CFTR to the plasma membrane) and VX-770 (also called Ivacaftor, a CFTR potentiator, that maintains the channel open in longer periods of time)
QUANTIFICAÇÃO DO TRANSPORTE IÓNICO UTILIZANDO A CÂMARA DE USSING
19 Outubro 2022, 16:00 • Ines Pankonien
· Ussing Chamber as a technique to study cellular integrity and the role of ion channels in epithelial transport
· Measure ion transport through Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) using the electrophysiological method Ussing chamber
· Study CFTR function in the cell line CFBE (Cystic Fibrosis Bronchial Epithelial cells) with wt- and F508del-CFTR expression
· Mutations in the CFTR gene lead to the disease Cystic Fibrosis
· Study how mutations in CFTR affect this channel activity
· Analyze the effect of pharmacological drugs in the rescue of these mutations, and show how the use of electrophysiology/Ussing Chamber can be used to predict the best drug for a patient with Cystic Fibrosis
· Pharmacological drugs used are VX-661 and VX-445 (also known as Tezacaftor and Elexacaftor, two CFTR correctors, that help to transport CFTR to the plasma membrane) and VX-770 (also called Ivacaftor, a CFTR potentiator, that maintains the channel open in longer periods of time)
Sistema Nervoso | Nervous system
19 Outubro 2022, 15:00 • Carlos Gravato
1- Sistemas de integração: células nervosas e células endócrinas
2- Neurónios
A estrutura celular e as regiões funcionais
Tipos de neurónios
3- Glia
4- Permeabilidade seletiva de membrana e potencial de membrana
1- Integrating Systems: nerve cells and endocrine cells
2- Neurons
The cellular structure and functional regions
Types of neurons
3- Glial cells
4- Membrane selective permeability and membrane potential
QUANTIFICAÇÃO DO TRANSPORTE IÓNICO UTILIZANDO A CÂMARA DE USSING
19 Outubro 2022, 12:30 • Ines Pankonien
· Ussing Chamber as a technique to study cellular integrity and the role of ion channels in epithelial transport
· Measure ion transport through Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) using the electrophysiological method Ussing chamber
· Study CFTR function in the cell line CFBE (Cystic Fibrosis Bronchial Epithelial cells) with wt- and F508del-CFTR expression
· Mutations in the CFTR gene lead to the disease Cystic Fibrosis
· Study how mutations in CFTR affect this channel activity
· Analyze the effect of pharmacological drugs in the rescue of these mutations, and show how the use of electrophysiology/Ussing Chamber can be used to predict the best drug for a patient with Cystic Fibrosis
· Pharmacological drugs used are VX-661 and VX-445 (also known as Tezacaftor and Elexacaftor, two CFTR correctors, that help to transport CFTR to the plasma membrane) and VX-770 (also called Ivacaftor, a CFTR potentiator, that maintains the channel open in longer periods of time)