Sumários
Polysaccharides - their structure and biological role
4 Novembro 2016, 14:00 • Amélia Pilar Grases dos Santos Silva Rauter
Introduction to polysaccharides. Dextran, glycosaminoglycans, proteoglycans - structure and biological role. Structural analysis of polysaccharides. The classical method and the Gary Gray approach. Revision of the lectured subjects.
Pathophysiological mechanism and glycosylation
31 Outubro 2016, 09:00 • Amélia Pilar Grases dos Santos Silva Rauter
Glycans in Glycobiology. The role of glycosylation in biological processes. Proteins recognizing glycans, physiological and pathological mechanisms - infection, immune response and cancer.
Aula adiada para o dia 16 de dezembro das 17h30 às 19h (2º teste de avaliação parcial)
28 Outubro 2016, 16:00 • Amélia Pilar Grases dos Santos Silva Rauter
Aula adiada para o dia 16 de Dezembro -2º teste de avaliação parcial.
NMR-guided approaches to decipher carbohydrate-protein interactions
28 Outubro 2016, 14:00 • Amélia Pilar Grases dos Santos Silva Rauter
NMR methods and molecular modeling protocols to disclose the features that govern glycan-receptor interactions. NMR Analysis in ligand-protein Interactions. Slow and fast brownian motion. Structural perturbations are reflected by changes in a variety of NMR observable sincluding: chemical shifts, NOEs, coupling constants and diffusion coefficients. Use of line broadening to detect ligand binding. Diffusion editing experiments. Saturation Transfer Difference NMR (STD-NMR) .TR-NOESY and the bioactive conformation. Combining NMR data with Molecular Modelling. Case studies.
Literature search and analysis of papers
21 Outubro 2016, 16:00 • Amélia Pilar Grases dos Santos Silva Rauter
Literature search and selection of papers for the critical review/seminar, within the topic chosen.